Dr. Stan did his postdoctoral training 1994-1999 at University of California San Diego Medical in the laboratory of George Palade, where he worked on the role of caveolae/lipid rafts and fenestrae in the cell biology of vascular permeability. He joined the faculty at UCSD as a Project Scientist (1999 ) and Research Assistant Professor (2000) in the Department of Cellular and Molecular Medicine continuing his studies of the molecular mechanisms of vascular permeability and the structures involved. In 2004, Dr. Stan joined the faculty of the departments of Pathology, and of Microbiology and Immunology at Dartmouth Medical School as an Assistant Professor and became Associate Professor in 2009. Currently, he is a member of the Heart and Vascular Research Center, Norris Cotton Cancer Center and affiliated with the Immunology COBRE. Since 2013 Dr Stan serves as the Director of the Optical Cell Imaging Facility and Co-Director, Irradiation, Pre-clinical Imaging and Microscopy Shared Resource, Norris Cotton Cancer Center.
Director, Optical Cell Imaging Facility, Co-Director, Irradiation, Pre-clinical Imaging and Microscopy Shared Resource, Norris Cotton Cancer Center, Lebanon, NH
Our laboratory studies the cell biology of vascular endothelial cells and their function in normal undisturbed tissues and in adaptive and pathologic processes underlying cancer and inflammatory diseases.
Our efforts are currently focused on several distinct but interactive project areas, relying on a wide array of experimental approaches including biochemical, cell biological, genetic and whole animal physiology approaches and state of the art fluorescence live cell imaging and electron microscopy. These focus areas are:
1) Cell biology of the endothelium: The laboratory studies how, on one hand, fundamental mechanisms of signaling and membrane traffic control endothelial cell differentiation and organization in different vascular beds. Specific endothelial structures (fenestrae, transendothelial channels, vesiculo-vacuolar organelles, caveolae and other vesicular carriers) have been shown to mediate the exchanges between blood and tissues. Our laboratory has cloned and characterized Plasmalemma Vesicle Associated Protein (PLVAP) gene, whose gene product, the PLVAP/PV1 protein, is the first known component of the endothelial stomatal and fenestral diaphragms, present on the endothelial structures involved in permeability. Taking advantage of PV1 we are currently trying to understand the biogenesis, cellular function and regulation of the endothelial structures involved in transendothelial exchange.
2) Pathways of exchange between blood and the interstitial space: We are particularly interested in elucidating the molecular mechanisms involved in the transendothelial exchange between the blood plasma and the interstitial fluid in health and disease. We are using genetically engineered mice generated in the lab to understand the precise role of vesicular trafficking as well as the role of endothelial microdomains such as transendothelial channels, fenestrae and vesiculo-vacuolar organelles in microvascular permeability.
3) Role of PV1 in inflammation: PV1 has exciting novel functions in the host immune response controlling diverse aspects of the immune system development (macrophage seeding of tissues and lymph node morphogenesis) and function (controls diapedesis of leukocytes into inflammatory lesions and soluble antigen entry into the lymph node conduits with repercussion on antigen processing and presentation). We are currently trying to understand the role of PV1 in immunity at molecular level and whether its biology can be harnessed for development of novel therapeutics and diagnostics.
4) Role of PV1 in cancer: Previous work has shown that PV1 is expressed in most, if not all, solid tumor vessels. We have shown that intratumoral deletion of PV1 results in impaired tumor growth, which prompted studies as to the precise mechanism by which PV1 promotes tumor formation and growth.
1. Determine the role of PV1 in inflammation. 2. Determine the role of PV1 protein in cancer. We are employing multiple cancer models to study the role of PV1 in tumor growth. 3. Development of anticancer diagnostics and therapeutics. 4. Characterize the role of PV1 and its interacting partners in the biogenesis and function of caveolae and fenestrae. Determine the roles of these endothelial structures in vivo.
Dr. Stan is accepting rotation students in the fall, winter and spring terms and is available as a thesis mentor for 2016-17.
General Pathology DMS I PEMM 101 PEMM 102 PEMM 275 PEMM 126 MICRO144
M.D. 1993 - Cluj, Romania Postdoctoral, 1994-1999 at University of California San Diego Medical (Advisor George E. Palade) Ph.D. 2013 - Groningen, The Netherlands
Geisel School of Medicine at Dartmouth, Department of Biochemistry and Cell Biology